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J Radiat Prot > Volume 10(1);1985 > Article
Journal of Radiation Protection 1985;10(1):29.
방사선(放射線)이 생쥐생식세포(生殖細胞)에 미치는 영향(影響)에 관(關)한 연구(硏究)
Studies of the Radiation Effects on Mouse Germ Cell
Chung, Kyu-Hoi;Chun, Ki-Jung;Chung, Hai-Won;Yoo, Byung-Sun;Lee, Jeong-Ho;
The objectives of present study is to investigate genetic damage of radiation in mammalian male germ cell and. to establish available screening method for determining genetic hazard by radiation. Several methods were employed to measure the genetic damage of radiation as follows: Sperm head counts, frequency occurrence of sperm with abnormal head shape, fertility, activity of LDH-X, and the induction of unscheduled DNA synthesis (U.D.S.) in male mouse were performed with the passing of time after irradiation by making use of the sequence of event that occurs during spermatogenesis. Sperm head counts and activity of LDH-X in testes were gradually reduced by increased radiation dose and with the passing of the time after irradiation. Frequency occurrence of sperm with abnormal head shape, sterile period, and the induction of unscheduled DNA synthesis were increased by increased radiation dose. It is suggested that since germ cell is a direct reflection of genetic complement, the use of male germ cell is rapid and convenient method for measuring genetic damage by radiation.
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