A deuterium oxide leakage accident occurred on October 4, 1999, at nuclear power plant in Korea. The concentration of tritium in air increased and 22 workers were exposed by tritium at that time. It is well known that tritium causes internal exposure. Therefore, we examined complete blood cell count, physical and biological dosimetry fur 13 workers among whole 22 workers to check the health effect and to evaluate the dose estimation of tritium exposure. The leukocyte count test, one of general blood test, was normal. The estimated doses were 0 - 4.44 mSv by physical dosimetry and 0-37 mGy by biological dosimetry. This dose does not exceed radiation dose limit, and the clinical symptoms of the exposed workers were not shown. The consistency between clinical sign and estimated dose means that physical and biological dosimetry were very useful especially in accident evaluation.