| 자외선 B 조사 마우스에서 표피멜라닌세포 변화에 대한 헤모힘의 방어효과 |
| 이해준;김종춘;문창종;정우희;박혜란;조성기;장종식;김태환;김성호; |
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| Protective Effect of HemoHIM on Epidermal Melanocytes in Ultraviolet-B irradiated Mice |
| Lee, Hae-June;Kim, Jong-Choon;Moon, Chang-Jong;Jung, U-Hee;Park, Hae-Ran;Jo, Sung-Kee;Jang, Jong-Sik;Kim, Tae-Hwan;Kim, Sung-Ho; |
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| ABSTRACT |
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We induced the activation of melanocytes in the epidermis of C57BL/6 mice by ultraviolet-B (UV-B) irradiation, and observed the effect of an herbal preparation (HemoHIM, HH) on the formation, and decrease of UV-B-induced epidermal melanocytes. C57BL/6 mice were irradiated by UV-B $80;mJ{cdot}cm^{-2}$ ($0.5;mW{cdot}sec^{-1}$) daily for 7 days, and HH was intraperitoneally, orally or topically applied pre- or post-irradiation. For the estimation of change of epidermal melanocytes, light microscopic observation with dihydroxyphenylalanine (DOPA) stain was performed. Split epidermal sheets prepared from the ear of untreated mice exhibited 13~15 melanocytes${cdot}mm^{-2}$, and one week after UV irradiation, the applied areas showed an increased number of strongly DOPA-positive melanocytes with stout dendrites. But intraperitoneal, oral or topical treatment with HH before each irradiation interrupted UV-B-induced pigmentation and resulted in a marked reduction in the number of epidermal melanocytes as compared to the number found in UV-B-irradiated, untreated control skin. The number and size of DOPA-positive epidermal melanocytes were also significantly decreased in intraperitoneally injected or topically applicated group after irradiation with HH at 3rd and 6th weeks after irradiation. The present study suggests the HH as inhibitor of UV-B-induced pigmentation, and depigmenting agent. |
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