| Home | E-Submission | Sitemap | Contact Us |  
top_img
J Radiat Prot > Volume 23(2); 1998 > Article
Journal of Radiation Protection 1998;23(2):89-0.
난포성숙호르몬이 감마선 조사된 미성숙 생쥐 난포에 미치는 영향
김진규;이창주;이영근;송강원;윤용달;
Effects of Follicle Stimulating Hormone on ${gamma}$-Ray Irradiated Immature Mouse Ovarian Follicles
Kim, Jin-Kyu;Lee, Chang-Joo;Lee, Young-Keun;Song, Kang-Won;Yoon, Yong-Dal;
ABSTRACT
To assess the radioprotective effects of follicle stimulating hormone (FSH) on ovarian follicles, 3 week-old female mice were irradiated with 8.33 Gy of ${gamma}$-ray (group R) and followed by 5 IU ip-injection of FSH (group RF). For control groups, 5 IU of saline (group C) or 5 IU of FSH (group F) was ip-injected. Ovaries were collected 0h, 6h, 12h, 14, 2d, 4d, and 8d after irradiation or saline/FSH injection, and followed by fixation in neutral buffered formalin for routine histochemistry. Immunohistochemistry was used to assess the status of follicles and DNA fragmentation was analyzed by agarose gel electrophoresis for total DNA. Staining specific for apoptotic follicles showed high intensity at 6h and 12h in group R and RF On the other hand, staining specific for proliferating follicles showed noticeably high intensity at 8d in group R and Rf. DNA fragmentation of 185bp increased with time in all experimental groups. Especially 370bp appeared at 6h in group R, then disappeared after 1d. In case of group RF, it appeared at 12h and disappeared after 1d. From the above results, the irradiated antral follicles become completely disappeared from 4d to 8d, and then new follicles started to grow again at 8d. FSH had delaying or suppressing effects on follicular atresia after irradiation. In addition, it became clear that radiation-induced follicular atresia was mediated by granulosa cell apoptosis.
TOOLS
PDF Links  PDF Links
Full text via DOI  Full text via DOI
Download Citation  Download Citation
Share:      
METRICS
1,150
View
1
Download
Cell cycle evaluation of granulosa cells in the $\gamma$-irradiated mouse ovarian follicles  1999 ;24(1)
Editorial Office
#319, Hanyang Institute of Technology Bldg., 222 Wangsimni-ro, Seongdong-gu,Seoul, Republic of Korea
Tel: +82-2-2297-9775   Fax: +82-2-2297-9776
Email: managing.editor@jrpr.org
About |  Browse Articles |  Current Issue |  For Authors and Reviewers
Copyright © by Korean Association for Radiation Protection. Developed in M2PI