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J. Radiat. Prot. Res > Volume 40(1); 2015 > Article
Journal of Radiation Protection and Research 2015;40(1):10-0.
doi: https://doi.org/10.14407/jrp.2015.40.1.010
성장기 마우스에서 방사선 유도 골소실 동물모델 확립
Establishment of a Murine Model for Radiation-induced Bone Loss in Growing C3H/HeN Mice
Jang, Jong-Sik;Moon, Changjong;Kim, Jong-Choon;Bae, Chun-Sik;Kang, Seong-Soo;Jung, Uhee;Jo, Sung-Kee;Kim, Sung-Ho;
Bone changes are common sequela of irradiation in growing animal. The purpose of this study was to establish an experimental model of radiation-induced bone loss in growing mice using micro-computed tomography (${mu}CT$). The extent of changes following 2 Gy gamma irradiation ($2Gy{cdot}min^{-1}$) was studied at 4, 8 or 12 weeks after exposure. Mice that received 0.5, 1.0, 2.0 or 4.0 Gy of gamma-rays were examined 8 weeks after irradiation. Tibiae were analyzed using ${mu}CT$. Serum alkaline phosphatase (ALP) and biomechanical properties were measured and the osteoclast surface was examined. A significant loss of trabecular bone in tibiae was evident 8 weeks after exposure. Measurements performed after irradiation showed a dose-related decrease in trabecular bone volume fraction (BV/TV) and bone mineral density (BMD), respectively. The best-fitting dose-response curves were linear-quadratic. Taking the controls into accounts, the lines of best fit were as follows: BV/TV (%) = $0.9584D^2-6.0168D+20.377$ ($r^2$ = 0.946, D = dose in Gy) and BMD ($mg{cdot}cm^{-3}$) = $8.8115D^2-56.197D+194.41$ ($r^2$ = 0.999, D = dose in Gy). Body weight did not differ among the groups. No dose-dependent differences were apparent among the groups with regard to mechanical and anatomical properties of tibia, serum ALP and osteoclast activity. The findings provide the basis required for better understanding of the results that will be obtained in any further studies of radiation-induced bone responses.
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